| 1 | Recognize the basic concepts of plant cell and tissue culture and explain its historical development. |
| 2 | Explain totipotency and different culture techniques (callus culture, organogenesis, embryogenesis, etc.) and give examples. |
| 3 | Use the equipment, tools, and sterilization techniques required for tissue culture. |
| 4 | Apply the processes of callus induction, organogenesis, and somatic embryo formation. |
| 5 | Explain and apply micropropagation techniques. |
| 6 | Evaluate secondary metabolite production through cell culture techniques. |
| 7 | Compare and discuss protoplast isolation and fusion techniques. |
| 8 | Design experiments, collect data, and analyze the obtained results. |
| 9 | Criticize and evaluate the application areas of plant tissue culture in biotechnology. |