1 | Overal introduction: Why serological and molecular techniques are necessary in detection and identification of plant pathogens? | [1]p172-174, [4]p1-3 |
2 | The information flow in plant cells: nucleic aids, DNA replication and protein synthesis | [2]p151-183 |
3 | Purification of pathogens. | [4]p4-10 |
4 | Production of polyclonal and monoclonal antisera | [3]p481-525 |
5 | DNA/RNA extraction from tissues | [4]p11-30 |
6 | Construction of gene libraries and gene mapping strategies | [4]p31-40 |
7 | Southern, Nothern and Western hybridisation methods | [4]p41-45 |
8 | Basic principles of polymerace chain reaction | [4]p45-55 |
9 | Selection of primers specific to a pathogen and primer design | [4]p55-59 |
10 | Multiplex PCR, RAPD, PCR-RFLP | [4]p59-62 |
11 | Arbitrary Fragment Length Polymorphisims, AFLP, Microsatellit (variable tandem repeats) DNA method | [4]p62-67 |
12 | Micro array techniques, Real-Time PCR (Sybergreen, TaqMan methods) | [4]p67-75 |
13 | DNA cloning | [4]p75-83 |
14 | DNA sequencing | [4]p83-90 |