PPT6117 / DETECTION METHODS OF PLANT PATHOGENS

DERSİN HAFTALIK İÇERİĞİ

 
KONULAR 
KAYNAKLAR 
1Overal introduction: Why serological and molecular techniques are necessary in detection and identification of plant pathogens?[1]p172-174, [4]p1-3
2The information flow in plant cells: nucleic aids, DNA replication and protein synthesis[2]p151-183
3Purification of pathogens.[4]p4-10
4Production of polyclonal and monoclonal antisera[3]p481-525
5DNA/RNA extraction from tissues[4]p11-30
6Construction of gene libraries and gene mapping strategies[4]p31-40
7Southern, Nothern and Western hybridisation methods[4]p41-45
8Basic principles of polymerace chain reaction[4]p45-55
9Selection of primers specific to a pathogen and primer design[4]p55-59
10Multiplex PCR, RAPD, PCR-RFLP[4]p59-62
11Arbitrary Fragment Length Polymorphisims, AFLP, Microsatellit (variable tandem repeats) DNA method[4]p62-67
12Micro array techniques, Real-Time PCR (Sybergreen, TaqMan methods)[4]p67-75
13DNA cloning[4]p75-83
14DNA sequencing[4]p83-90