FACULTY OF AGRICULTURALSCIENCES AND TECHNOLOGIES / AGRICULTURAL GENETIC ENGINEERING / TGM2010 - PLANT CELL, TISSUE AND ORGAN CULTURE

Contents Of The Courses in a weekly Period

Week 
Subjects 
Sources 
1Introduction to plant tissue culture, its history 
2Organization of a plant tissue culture lab; sterilization and laboratory applications 
3Maintaining aseptic conditions, preparation of culture media and their properties 
4Introduction to biosafety levels; types of biosafety cabinets 
5Introduction to different explant used in invitro culture; regeneration types 
6Introduction to various plant growth regulators 
7Midterm Exam 
8Applications of Cytokinin in tissue culture experiments 
9Use of auxin, gibberelins and other growth regulators in plant tissue culture 
10Basics of Gene cloning 
11Gene Cloning continued..... 
12Introduction to Genetic transformation technologies 
13Types of genetic transformation continued.......... 
14Role of plant tissue culture in sustainable agriculture